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KMID : 0390119940340020205
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Journal of Pusan Medical College
1994 Volume.34 No. 2 p.205 ~ p.214
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Phorbol 12, 13-dibutyrate-induced Contraction of Canine Basilar Artery
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Abstract
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This study was inmd to characerize phorbol 12, 13-dibutyrate (PDBu)=induced contracton of acnine absilar artery. The arterial rings were myograied isometrially in an isolated organ bath.
1. PDBu, an activary of protien kinese C, induced slow-developing ustained contractions in ring of canine basilar artery in a dose dependent manner. PDBr-induced contraction was virtually inhibited by staurosporine which is known as a protein
kinase C
inhibitor.
2. PDBu 10 nM. A subthreshold concentration of contraction. reduced phenylephrine-induced contraction and ehnanced hig K-induced contraction.
3. PDBu enhanced hig K-induced contraction.
4. In CA-free solution. PDBu induced a sustained conraction even after internal Ca2+ pool was exhausted by phenylephrine. But phorbol 12-nyristate 13-acetate (PMA) did not induce tensile reponse.
5. IBMX and papaverine, phosphodiesterase inhibitors, reduced PDBu-induced contraction. PDBu-induced contraction was reduced by 8-bromo-cGMP, a permeable analogue of cyclic nucleotide, but not by 8-bromo-cAMP. Methylene blue, a inactivator of
guanylate
cyclase, potentiated PDBu- and phenylephrine-induced contraction.
There result suggest that in canine bsilar artery PDBu-induced contraction could be mediated by the activation of protein kinase C, and endothelial function is important in the regulation of sustained vasospasm.
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KEYWORD
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